Its equipment resources are extensive and include cell and tissue culture, fluorescence microscopy, recombinant DNA cloning and sequencing, recombinant protein purification, protein sequencing, peptide synthesis, mass spectroscopy, and advanced computers.
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Objective To develop a method for the purification of recombinant human collagen-like protein.
目的建立重组类人胶原蛋白分离及纯化工艺。
The paper describes process of recombinant fusion protein in purification and existing problems.
本文论述了基因重组融合蛋白纯化过程和存在的问题。
RESULTS: After purification the form of soluble mutant increased remarkably but the forms of dimmer and higher multimer were reduced greatly to no more than 8% of the total recombinant protein.
结果：纯化后突变蛋白的可溶性成分大幅度增加，而二聚体与多聚体显著减少至纯化后总蛋白的8 %以下。
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