Objective To develop a method for the purification of recombinant human collagen-like protein.
目的建立重组类人胶原蛋白分离及纯化工艺。
The paper describes process of recombinant fusion protein in purification and existing problems.
本文论述了基因重组融合蛋白纯化过程和存在的问题。
RESULTS: After purification the form of soluble mutant increased remarkably but the forms of dimmer and higher multimer were reduced greatly to no more than 8% of the total recombinant protein.
结果：纯化后突变蛋白的可溶性成分大幅度增加，而二聚体与多聚体显著减少至纯化后总蛋白的8 %以下。
To construct the recombinant prokaryotic expression vector containing the ESAT-6 gene of Mycobacterium Boris, purification, expression the fusion protein identified.
构建牛结核分枝杆菌esat - 6基因的原核表达载体，诱导表达、纯化并初步鉴定该蛋白。
To construct the recombinant prokaryotic expression vector containing the ESAT-6 gene of Mycobacterium Boris, purification, expression the fusion protein identified.
构建牛结核分枝杆菌esat - 6基因的原核表达载体，诱导表达、纯化并初步鉴定该蛋白。
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