Objectives To establish an nested (polymerase chain reaction) PCR analytic method with restrictive enzyme to detect human cytomegalovirus (HCMV) rapidly in clinics.
目的建立套式聚合酶链反应（PCR）加限制性酶切分析方法检测人巨细胞病毒（HCMV）。
One step nested reverse transcriptase-polymerase chain reaction (RT-PCR) was used.
使用一步法巢式逆转录聚合酶链扩增法(RT - PCR)。
Objective To study the sensitivity and specificity of single tube nested polymerase chain reaction (SN PCR) technique in detecting Mycobacterium tuberculosis DNA in paraffin embedded tissues.
目的探讨单管巢式聚合酶链反应(SNPCR)检测石蜡包埋组织结核分支杆菌dna的特异性和敏感性。
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