Methods an Alkaline lysis method was used to isolate serum HBV-DNA for fluorescence quantitative PCR and compared with conventional phenol-chloroform extraction method and boiling lysis method.
方法使用碱裂解法、酚氯仿法和煮沸裂解法同时提取血清hbv - DNA，比较荧光定量pcr测定的重复性和测定值的差异。
Compared with conventional method, the present method can be readily used for clinical detection as it only requires microliters of serum and does not need ultracentrifugation for isolation of VLDL.
本法与常规法比较。不需要超速离心分离人血清VLDL的过程，而且仅需微量血清，便于临床应用。
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