On the basis of orthogonal design and colonial diameter as mutation target, the mutagenesis effect of NTG on Pleurotus sp. 8405 is studied.

The effects of irradiation of UV rays on the growth of hyphae of Morchella esculenta , Trichoto-ma matsutake and Collybia velutipes were studied on potato culture media.

The experimental results showed that the actions of all the five factors to the bio wave growth were extremely remarkable, and the order was agar, peptone, sucrose, beef extract, yeast extract.