A gas chromatographic method for the quantitative analysis of monopalmitin and monoolein in fat and oil was described.

Monoglycerides, sugar esters, polyglyceryl fatty esters and long chain fatty wax esters are important biosurfactants.

The combined Fatliquor LWF is prepared by methyl ester which was synthesized through the alcoholysis reaction and catalysised by anion layer and pillar materials with deteriorated oil.