Methods: the multiple proportion dilution of the above mentioned compounds were selected and used in vitro experiment.
2
方法:采用稀释法用生理盐水将不同型别的霍乱弧菌进行倍比稀释,观察其在庆大霉素琼脂平皿上的生长情况。
Methods: Double ratio dilution method by physiological saline was applied to different type of Vibrio cholera, and their growth on gentamicin AGAR plate was observed.
Then standard curve of ATP were drawed. Tumor cell lines H1299 were serial diluted and their ATP were measured by reaction solution prepared to analyse the minimum cell number.