Sometimes a blunt cut is useful if you want to sort of grow a sticky end of your choice on it.

这时候钝切口很有用,尤其在你想获得选定的粘性末端的时候

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"Man's heart" in Yeats is "resinous"; it's a sticky filth that flames.

人心于叶芝看来是树脂做的;,是粘乎乎会燃烧的脏东西。

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When it cuts it leaves sticky ends or un-base paired single stranded regions on each end of the part its cut and that's just a property of many restriction enzymes; not all, some cut blunt,just right down the middle.

它切割后会留下两个粘性末端,或者未配对的单链部分,这是许多限制性内切酶的特性,但不是全部,有些限制性内切酶是从中间钝切的

耶鲁公开课 - 生物医学工程探索课程节选

If I cut both the plasmid and my DNA of interest with the same restriction enzyme I'm going to end up with the same sticky ends on both molecules.

如果用同一种限制性内切酶,来切割质粒和我感兴趣的DNA,在两个分子上就能得到同样的粘性末端

耶鲁公开课 - 生物医学工程探索课程节选

If you cut and you open up a segment of DNA then you've left these sticky ends, for example and these sticky ends are capable of recognizing each other by the process of hybridization.

如果你进行切割打开一个DNA片段,就会留下,比如说,这些粘性末端,这些粘性末端能在杂交的过程中互相识别

耶鲁公开课 - 生物医学工程探索课程节选

Now if I put them in contact with one another, the plasmid that's been opened and fragments of the DNA - special fragments that I've produced with the same restriction enzyme, they'll have the same sticky ends, they will naturally hybridize with one another.

如果我让它们互相接触,已经切开的质粒和DNA片段--,这DNA片段是我已用同种限制性内切酶,处理过的特别片段,这两者会有相同的粘性末端,它们会自然地相互杂交

耶鲁公开课 - 生物医学工程探索课程节选