• 受体菌 - 引用次数：1

  The homologous recombinant fragments of Xna gene are amplified by the method of overlap extension PCR,The homologous recombinant vector is constructed successfully by cloning the amplified recombinant fragments to the suicide vector of pJQ200,and then transformed into X. nematophila var. pekingensis by two parent's conjunction. The conjunction condition optimization results show that the conjunction efficiency reaches the highest level when the ratio of donor bacterium of E. coli S17-1 and recipient bacterium of CB6 bacterium is 2 and conjunction time 8 hour.

  4.采用重叠延伸引物PCR法扩增了CB6菌株Xna基因的同源重组全长片段,并将该片段连接到自杀性载体pJQ200上,利用二亲本结合转移法成功转入CB6菌体中,并对转化效率进行了优化,结果表明:供体菌S17-1和受体菌CB6菌株的比例为2,结合时间为8小时时,转化效率最高,达到1.08×10~(-4)。

  参考来源 - 抗生素帕克素发酵条件的优化和合成相关基因的初步研究

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