Methods: VSMCs were cultured by tissue explants adherent method.

方法：采用组织块贴壁法培养大鼠主动脉平滑肌细胞。

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Artery and vein were removed and mesenchymal tissue was taken out. The primary cells were performed culture and purification with collagenase digestion method to obtain the adherent cells.

无菌条件下取正常足月剖腹产的脐带近胎儿段，剔除动静脉，取出其中的间质组织，胶原酶消化法原代培养细胞进行培养和纯化获得贴壁细胞。

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