Objective To establish a semi-quantitative method for measurement of HCV RNA by use of primers and probe, which was sensitive and designed by ourselves, a new europium fluorescent chelate BHHCT.

目的利用自行设计的引物、探针和新型铕螯合物BHHCT，建立一种半定量丙型肝炎病毒(HCV) RNA的检测方法。

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Methods Semi-quantitative PCR method was used in this research to estimate PERV copy-number of four mini-pigs in China.

方法采用半定量pcr方法检测了我国四种小型猪内源性逆转录病毒基因的拷贝数。

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Based on improvement of fuzzy qualitative simulation algorithm, a new semi-quantitative and real-time fault prediction method of a class of dynamic system with incomplete information is proposed.

在对模糊定性仿真算法改进的基础上，提出了一类信息不完整动态系统的实时半定量故障预报方法。

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