This research intended to construct a eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene.

本研究旨在构建具有猪 MSTN 前肽基因定点突变的真核表达载体。

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The disease has a familial hereditary tendency, genetic research shows a non-singleness genetic character, and the manifestations of its pathogenic gene and mutation site are various.

本病有家族遗传倾向，遗传学研究呈非单一性遗传特点，其致病基因及突变位点表现多样。

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It is also improved M and NS genes by a site mutation and a gene deletion method.

通过点变异和基因删除方法对M和NS基因进行改进；

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