试验中利用前人已报告对 potyviral RNA之3' 端核甘酸序列具广效性之引子对 (degenerate primers) ( 1 5 ) ，进行反转录—聚合脢连锁反应 (reverse-transcription polymerase chain reaction, RT- P C R )， 将TMMV RNA 3' 端涵...
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degenerate primers pcr 简并引物pcr
Special degenerate primers 特异性简并引物
degenerate primers polymerase chain reaction 简并引物pcr
METHODS Using degenerate primers designed to the conserved type Ⅲ PKS gene from plants and RACE technology, a PKS gene in Fallopia multiflora was cloned. Its characteristics were analyzed by RT-PCR and bioinformatics analysis.
方法根据已知PKS基因的保守序列设计简并引物,RACE技术克隆何首乌中的PKS基因;利用生物信息学工具分系其生化特征,RT-PCR分析其在各组织中的表达差异。
参考来源 - 何首乌Ⅲ型PKS基因的克隆和表达分析·2,447,543篇论文数据，部分数据来源于NoteExpress
In the PCR reactions, the coneen- tration ratio between the degenerate primers was essential for influencing amplification efficiency.
在PCR反应中，简并引物之间的浓度配比是影响扩增效率的关键。
A pair of degenerate oligonucleotide primers named L2R2were synthesized according to conserved regions of the cytadhesin proteins from M. pneumonias. MG-S6DNA were used as model in L2R2 primered PCR.
根据肺炎支原体细胞粘附素蛋白质的基因保守区两侧序列，设计并合成一对变性寡核苷酸引物L2R2，用于以鸡毒支原体S6株基因组DNA为模板的PCR反应。
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