• While he acknowledges that there is much to be learned from alternative research methods like cell culture a process by which cells are taken from a living organism and grown under controlled conditions VandeBerg says they have learned a lot more from research on chimpanzees.

    VOA: standard.2010.02.16

  • Now, you know, because we talked about cell culture already, that a great--that we talked about last week, manufacturing of cells.

    你知道,因为我们已经讲过细胞培养,我们上周讲过的,细胞的大规模制造

    耶鲁公开课 - 生物医学工程探索课程节选

  • We'll talk about cell culture engineering during Week 4, how do you maintain cells in culture, what are the limits of this.

    第四周我们会探讨细胞培养工程,人们如何在培养基中维持细胞的存活,这种方法的局限性是什么

    耶鲁公开课 - 生物医学工程探索课程节选

  • Another comparison for a meaningful drug screening essay, I would assume you would need 1 million cells that you could grow easily in a standard cell culture dish.

    我们不妨再用一个,便于你理解的药物筛选试验来打个比方,假设正常情况下,一个标准细胞培养皿需要一百万个细胞。

    斯坦福公开课 - 7个颠覆你思想的演讲课程节选

  • We talked about attenuating in the laboratory, using cell culture techniques and what we know about mutating viruses.

    我们讲过实验室中,利用细胞培养技术培养减毒疫苗,以及我们所了解的减毒病毒

    耶鲁公开课 - 生物医学工程探索课程节选

  • You could use cell culture, so you don't have to grow the virus naturally in animals.

    你可以利用细胞培养技术,那你就不必在动物身上培养病毒

    耶鲁公开课 - 生物医学工程探索课程节选

  • One of the purposes of cell culture is to make many, many more cells under controlled conditions where I can understand what those cells are.

    细胞培养的一个目的就是,在可控的环境下生成许许多多的细胞,从而让我能够研究这些细胞

    耶鲁公开课 - 生物医学工程探索课程节选

  • Well, it turns out that we could not identify, at least at that point, a cell culture that would serve as a host for vaccinia.

    然而我们还不能断定,至少这个时候无法断定,这些被感染的细胞就是牛痘病毒的宿主

    耶鲁公开课 - 生物医学工程探索课程节选

  • One of the main things that we use cell culture or maintenance of cells outside the body for is to make more copies of cells.

    我们培养细胞,或者体外维持细胞的重要步骤,就是复制更多的细胞

    耶鲁公开课 - 生物医学工程探索课程节选

  • In particular, cell culture technology or how you culture cells outside the body.

    特别是,细胞培养技术,换句话说,就是如何在体外培养细胞

    耶鲁公开课 - 生物医学工程探索课程节选

  • A group of scientists, mainly in Boston found, importantly, that they could cultivate the polio virus, the disease causing polio virus; they could cultivate it in cell culture.

    在波士顿有一队科学家有了重大发现,他们可以培养脊髓灰质炎病毒,也是就引发小儿麻痹症的病毒,他们可以通过细胞培养来孵育这种病毒

    耶鲁公开课 - 生物医学工程探索课程节选

  • It's a picture of cells actually growing and moving in cell culture.

    这幅图是真实细胞,在细胞培养液中生长和运动的样子

    耶鲁公开课 - 生物医学工程探索课程节选

  • Well, if you knew a cell that could serve as a host for a virus, you could use this process of cell culture to make large quantities of virus,right?

    如果你知道细胞可以变成病毒的宿主,可以用培养细胞的过程,制造大量病毒,对吧

    耶鲁公开课 - 生物医学工程探索课程节选

  • Cell proliferation is a huge issue in cell culture.

    细胞增殖是细胞培养中的重大课题

    耶鲁公开课 - 生物医学工程探索课程节选

  • Yeast cells were grown in large numbers with this plasmid inside, they expressed the plasmid and so you made Hepatitis B surface antigen not in people but in cell culture where it was not normally formed.

    体内携带这种质粒的酵母细胞大量增殖,它们表达质粒,因此,乙型肝炎表面抗原不是在人体内,而是在细胞培养环境下,以非正常方式形成的

    耶鲁公开课 - 生物医学工程探索课程节选

  • Ok, today we're going to continue our discussion of cellular principles and lead into cell culture technology which will be the subject of the section meeting this afternoon, so just to remind you about the sections.

    今天我们继续讨论细胞学原理,之后再引申到细胞培养技术,这也是我们今天下午分组讨论会的主题,顺便提醒一句,下午有组会

    耶鲁公开课 - 生物医学工程探索课程节选

  • This property of cells in culture is called anchorage dependence - that the cell needs to be anchored to a surface in order to function properly.

    体外培养细胞的这种特性,称为贴壁依赖性--,意思是细胞必须固定在,某个表面上才能正常生长

    耶鲁公开课 - 生物医学工程探索课程节选

  • The other pathway that I think is very promising is now to use stem cell generated cells in culture dishes to use them for high throughput drug screening now we can test thousands of different drugs without killing so many animals.

    第二个目标的前景相当广阔,我们可以用干细胞,在培养皿中大量繁殖各种细胞,用于大剂量的药物试验,让你无需屠戮动物,就能进行各种药物试验。

    斯坦福公开课 - 7个颠覆你思想的演讲课程节选

  • So cell culture production wasn't an option.

    因此把细胞作为病毒培养基行不通

    耶鲁公开课 - 生物医学工程探索课程节选

  • In Week 3 you'll actually do some cell culture in the laboratory and look at cultured cells and learn how to manipulate, do some manipulations on cells and culture, and so on throughout the weeks.

    第三周你们会在实验室里做一些细胞培养,看看培养基中的细胞都长什么样,了解细胞和培养基的有关操作,整个一周就做这些

    耶鲁公开课 - 生物医学工程探索课程节选

  • If we grew these cells on a plate loaded with Ampicillin and we could select cells that have Ampicillin resistance, and this process of selection and cell culture is very important and we'll talk about it more next week.

    如果我们在含有氨苄青霉素的,培养基上培养细胞,我们可以筛选出,对氨苄青霉素有抗性的细胞,这个筛选和培养细胞的过程是很重要的,我们下星期会讲到这些

    耶鲁公开课 - 生物医学工程探索课程节选

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