Several genes associated with iron acquisition or regulation of iron metabolism were identified and further analyzed using real-time rt-pcr.
几个相关基因的调控和铁收购或铁代谢进行识别与分析使用实时通过。
In the paper, the rapid and accurate methods for detection and identification of food borne pathogens were established using real time PCR, RT-real time PCR and DNA chip.
本文运用实时荧光pcr、实时荧光rt - PCR和DNA芯片技术建立了快速检测食源性致病菌的方法。
Objective To increase the sensitivity of quantitative detection of gene transcripts in peripheral blood using real time RT PCR, two specimen processing methods were compared.
目的选择合理的标本处理方法，提高外周血基因转录本检测的灵敏度。
We analyze some genes including cell cycle, apoptosis, stress and inflammation, which are confirmed by the real time RT-PCR, and the results are corresponded to that of cDNA microarray.
我们对部分细胞周期、细胞凋亡、细胞应激与炎症反应相关基因进行了分析并用实时荧光定量pcr进行了验证，证实了基因芯片结果的可靠性。
Then the GO and KEGG Pathway enrichment analysis were carried as well as the functional analysis and real time RT-PCR validation of two important genes.
通过表达谱芯片检测药物作用后的基因表达差异，对差异表达基因进行生物信息学研究并结合实时荧光定量pcr分析。
Myocardial ACE2 mRNA expression was respectively determined by real-time PCR (RT-PCR) in control group and diabetes group.
采用实时定量pcr (RT - PCR)检测正常及糖尿病大鼠心肌组织ace2的基因表达。
System real time quantitative RT-PCR technology and immunohistochemistry were used to detect the Chlamydia trachomatis urogenital tract, the expression of UU.
结果：①实验组女性泌尿生殖道沙眼衣原体、解脲支原体的表达高于对照组。
The real time RT-PCR revealed that expressions of FABP4 in longissimus muscle from 160 and 200-day's sheep were higher than those from 90-day's (P<0.05), expectively.
实时荧光定量PCR试验表明，FABP4基因在160和200日龄绵羊背最长肌中的表达量明显高于90日龄背最长肌的表达量（P< 0.05）；
The real time RT-PCR revealed that expressions of FABP4 in longissimus muscle from 160 and 200-day's sheep were higher than those from 90-day's (P<0.05), expectively.
实时荧光定量PCR试验表明，FABP4基因在160和200日龄绵羊背最长肌中的表达量明显高于90日龄背最长肌的表达量（P< 0.05）；
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