Results During long term cultivation, the population doubling time of the cells became shorter and the percentage of aneuploidy chromosomes and colonies grown in soft AGAR increased.
结果卵圆细胞在培养过程中倍增时间逐渐缩短，非整倍体染色体数目增加，在软琼脂中生长的克隆增多。
Results Population doubling time of corneal stromal cells prolonged significantly since passage 7. MTT assay also showed cell viability of passage 7 decreased to 60% of passgage 1 (P< 0. 01 ).
细胞群体倍增时间由第7代起显著延长（P<0.01）；MTT比色法的结果亦显示，第7代细胞的增殖能力开始大幅下降（P<0.01）。
Results Population doubling time of corneal stromal cells prolonged significantly since passage 7. MTT assay also showed cell viability of passage 7 decreased to 60% of passgage 1 (P< 0. 01 ).
细胞群体倍增时间由第7代起显著延长（P<0.01）；MTT比色法的结果亦显示，第7代细胞的增殖能力开始大幅下降（P<0.01）。
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