The sample is transported through the column by the mobile phase .
试样由移动相带着穿过色谱柱。
I know the general rule that approximately 10 column volumes of mobile phase is necessary for reequilibration, but I must demonstrate and validate that the reequilibration conditions are sufficient.
我知道通常的规则是需要约10个柱体积的流动相来重新平衡柱子，但是我必须要验证给别人看这些重新平衡条件是充分的。
The detection wavelength, sample preparation and the optimization of mobile phase constitution were discussed.
讨论了检测波长的选择和样品的制备，优化了流动相的组成。
In adsorption chromatography, solute molecules of the mobile phase undergo many successive adsorptions and desorptions.
在吸附色谱中，流动相的溶质分子进行多次连续的吸附和脱附。
Effects of ionization environment, including reaction ions type and amounts in APCI source, on ionization mode of anthraquinones were examined by changing the flow rate and mobile phase proportion.
通过改变流动相的流速和比例改变离子源中反应离子的种类和数量，研究离子化环境对蒽醌类化合物电离方式的影响。
In some cases, the sample may be diluted with mobile phase to improve the peakshape.
在一些情况下，为了改善峰形，样品溶液可以用流动相稀释。
Experimental results further showed that dead volumes were also related to factors, such as the characteristics of the mobile phase, its composition and the column temperature.
结果表明死体积与流动相种类、配比和柱温等因素有关。
By predicting the retention time, peak width and resolution of each solute under different mobile phase conditions, the overlapping separation range map of the solutes in the sample is plotted.
该方法通过预测不同流动相条件下各组分的保留时间、峰宽和分离度，绘制出对于样品中各组分的重叠分离区域图。
Methods Under linear scale up mode, overlapping separation range map was used to optimize the composition of mobile phase.
方法在线性放大模式下，使用重叠分辨图法对流动相组成进行优化调节。
Additionally, enthalpy and entropy both contributed to enantioseparation when mobile phase was acetonitrile.
流动相为纯乙腈时，焓和熵对对映体的拆分均有贡献。
Objective: to establish an HPLC method for the resolution of metoprolol enantiomer using chiral mobile phase additives.
目的：建立hplc手性流动相添加剂法拆分美托洛尔对映体的方法。
It is described that effect of resin type, mobile phase flow and temperature on separation.
探讨了树脂类型、洗脱剂流量和酸度及层析温度等对分离的影响。
The influence of types and concentration of ionic liquids, detection wavelength, proportion of mobile phase, flow rate on the result of detection were discussed.
探讨了包括离子液体探针试剂的种类、用量、检测波长、流动相比例、流速等对检测效果的影响。
The effects of mobile phase composition, flow rate, column temperature, gradient ramp, ph value, salt concentration, and buffer concentration on capacity factors (K ') were respectively discussed.
对流动相组成、流率、柱温、梯度速率、pH值、盐浓度以及缓冲液浓度对容量因子(K')的影响分别进行了讨论。
Chemical Separations can be accomplished using HPLC by utilizing the fact that certain compounds have different migration rates given a particular column and mobile phase.
使用HPLC，利用在给定的色谱柱和流动相下化合物具有不同移动速率的事实可以实现化学分离。
There are many advantages which the ordinary CEC method doesnt have by using the pure organic solvent as mobile phase.
它以纯有机溶剂作电色谱流动相，具有许多水溶液电色谱所不能比拟的优点。
Chloroform - tetrahydrofuran - water (500:500:8) was used as mobile phase for the gradient elution of other strongly retained substances.
继用氯仿-四氢呋喃-水(500：500：8)为流动相，梯度洗脱其他强保留组分。
METHOD HPLC gradient elution method was used with methanol-water as the mobile phase to determine the contents of triptolide in its root, branch and leaf at different periods of its storage.
方法以甲醇-水为流动相，采用HPLC梯度洗脱法，测定了雷公藤药材的根、枝、叶中雷公藤甲素在不同存贮期的含量。
The gradient elution is reasonable when the retention time is 2.3 times after the appearance of prominent peak with initial mobile phase acetonitrile-water (40:60).
用初始流动相乙腈-水(40：6 0)洗脱至主峰出峰保留时间的2.3倍时开始进行梯度洗脱比较合理。
The effects of temperature, flow rate and composition of mobile phase on retention, resolution, column efficiency and the pressure of the system were examined.
考察了温度、流速和流动相组成对酚类样品的保留、分辨、柱效和系统压力的影响，探讨了酚类样品在聚合物柱上的热力学行为。
The mobile phase consisted acetonitrile - water( 1:4).
以乙腈-水（1：4）为流动相；
The effects of composition of mobile phase, the structure of solute and the column temperature on chiral separation were extensively studied. The chiral recognition mechanism was discussed.
考察了流动相组成、溶质结构和柱温对对映体分离的影响，并初步探讨了手性识别的机理。
NH2 column was used with the mobile phase of acetonitrile-water (75:25). The differential refractive index detector was used.
采用氨基键合硅胶柱，以乙腈-水(75∶25)为流动相，使用示差折光检测器。
Study on HPLC method for analyzing of trace pigments in ready-to-drink tea was conducted by using different eluants with acetonitrile as mobile phase.
以含乙腈的流动相作为洗脱剂，对茶饮料中微量色素物质的HPLC分析方法进行了研究。
Study on HPLC method for analyzing of trace pigments in ready-to-drink tea was conducted by using different eluants with acetonitrile as mobile phase.
以含乙腈的流动相作为洗脱剂，对茶饮料中微量色素物质的HPLC分析方法进行了研究。
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