This research intended to construct a eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene.
本研究旨在构建具有猪 MSTN 前肽基因定点突变的真核表达载体。
The disease has a familial hereditary tendency, genetic research shows a non-singleness genetic character, and the manifestations of its pathogenic gene and mutation site are various.
本病有家族遗传倾向，遗传学研究呈非单一性遗传特点，其致病基因及突变位点表现多样。
It is also improved M and NS genes by a site mutation and a gene deletion method.
通过点变异和基因删除方法对M和NS基因进行改进；
These gene mutation distributing in all over the whole coding section of ATM gene. Every Exon exist the gene mutated site and no obvious hot mutated site be discovered.
这些突变分布于atm基因整个编码区，每个外显子都存在基因变异位点，没有发现明显的突变热点。
The P53 gene mutation site in osteosarcoma was mostly in GC sequence of exon, especially in exon 7, which was different with other tumors.
骨肉瘤p 53基因多位于外显子GC序列，尤其是外显子7，有别于其他肿瘤好发位点。
This research intended to construct eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene, and verify its expression efficacy in C2C12 cells.
本研究旨在克隆通城猪含有第1个内含子的MSTN前肽基因，构建真核定点诱变载体，并通过转染C2C12细胞验证载体表达的有效性。
Conclusions No functional FANCA protein was found in these 3 cases of FA-A, and intragenic deletion, frame shift and splice site mutation were the major pathogenic mutations found in FANCA gene.
结论3例FA-A型患者均无功能性FANCA蛋白表达；基因缺失、移码突变和剪切位点突变是FANCA基因的主要失活方式。
Methods PCR method was applied to detect the KDR mutation of the target site (sodium channel) gene.
方法采用聚合酶链反应(PCR)方法，用自行设计的特异引物，对昆虫的钠离子通道基因进行扩增。
Conclusion the site specific point mutation system can modify human gene in vitro more accurately. It is useful in the setting up of animal models.
结论体外定点突变系统可以对基因进行精细的修饰，为建立更精确地模拟人类疾病的动物模型打下基础。
Conclusion the site specific point mutation system can modify human gene in vitro more accurately. It is useful in the setting up of animal models.
结论体外定点突变系统可以对基因进行精细的修饰，为建立更精确地模拟人类疾病的动物模型打下基础。
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