Methods The serum samples from 310 cases of viral hepatitis were tested by Fluorescence quantitative PCR assay (FQ PCR), and also by ELISA as contrast.
方法运用荧光定量pcr (FQ - pcr)和el IS A两种方法同时检测了310份肝炎患者血清，并对结果进行了对比分析。
Conclusion FQ PCR is a method with high sensitivity, specificity, accuracy, efficiency and low cross contamination, and can be widely applied for diagnosis.
结论荧光定量pcr技术具有高灵敏性、高特异性、高精确性、效率高和污染小等特点，是一种有效、快速和简便的检测方法，具有广泛应用价值。
Objective to evaluate the clinical application of fluorescent quantification polymerase chain reaction (FQ PCR) to detect cytokeratin 19 (CK19) in the diagnosis of breast cancer.
目的探讨荧光定量pcr (FQ PCR)检测细胞角蛋白19 (ck19)在乳腺癌诊断中的应用。
Objective To investigate the fluorescence quantitative polymerase chain reaction (FQ-PCR) in the giant cell viral hepatitis in value.
目的探讨荧光定量聚合酶链反应(FQ - PCR)在巨细胞病毒性肝炎中的应用价值。
Real-time detection ensures accurate quantity, so FQ-PCR can be used to monitor the true state of HCMV infection and amplification.
FQ PCR可以准确定量检测HCMV的真实感染和复制情况，对于临床诊断与治疗有一定的指导意义。
Objective To evaluate the clinical value of fluorescence quantitative PCR(FQ-PCR) technique in diagnosing tuberculosis.
目的探讨荧光定量聚合酶链反应（FQ-PCR）技术诊断结核病临床应用价值。
Objective To investigate the clinical application of real time fluorescence quantitive Polymerase Chain Reaction (FQ-PCR) in detection of HCV RNA in serum and peripheral blood monocular cells (PBMC).
目的探讨实时荧光定量聚合酶链反应(FQ - PCR)在检测外周血清及单核细胞中hcvRNA含量的临床应用价值。
Objective To investigate the clinical application of the Fluorescence quantitative PCR (FQ-PCR) in the detection of HCV infection.
目的：用荧光定量聚合酶链反应(FQ - PCR)测定丙型肝炎病毒在临床的应用。
Objective: Detect the infection of CMV and TOX in tissue , serum of unexplained abortion by FQ-PCR.
目的探讨用荧光定量PCR检测不明原因流产组织及血清中巨细胞病毒（CMV）和弓形体（TOX）感染。
Objective To explore the sensitivity difference between fluorescent quantity PCR (FQ-PCR) and routine PCR for HBV DNA detection.
目的探讨荧光定量聚合酶链反应(FQ PCR)与常规聚合酶链反应检测乙型肝炎病毒(HBV)敏感性的差异。
Methods the secretion specimen coming from the high risk patients with CA were examined with fluorescent quantitative polymerase chain reaction (FQ-PCR) for genotype HPV-DNA.
方法应用荧光定量聚合酶链反应(FQ - PCR)对尖锐湿疣高危人群分泌物标本进行HPV - DNA分型检测。
Methods to construct the standard plasmid carrying HCMV-DNA; PCR and FQ-PCR techniques were used to detect the plasmid with different dilution.
方法构建携带CMV - DNA的标准质粒，利用常规pcr技术及FQ - P CR技术分别对不同稀释度的标准质粒进行检测。
Objective:To evaluate the clinical application value of FQ-PCR(fluorogenic quantitative polymerase chain reaction) for detecting HP DNA in human gastric mucosa.
前言： 目的：评估荧光定量PCR在检测胃粘膜上幽门螺杆菌的DNA中的应用价值。
Conclusion FQ-PCR, characterized by distinct specificity, high sensitivity, simplicity and quick-response, can be used as a conventional method for detecting MTB.
结论应用荧光定量pcr方法检测结核杆菌，具有特异、灵敏、简便、快速的特点，可作为结核病诊断的方法之一。
Liver function and the markers of HBV were detected. The contents of HBV- DNA in serum and in gastric mucosa were assayed respectively by fluorescence quantitative polymerase chain reaction (FQ-PCR).
用核酸扩增荧光定量法检测血清、胃黏膜HBVDNA ，综合分析各检测值对肝胃不和证积分的意义。
Method: Collected throat swab of influenza-like cases, extracted viral RNA in throat swab specimens and detected the viral RNA by FQ-PCR.
方法：采集流感样病例咽拭子，提取标本中的病毒核酸，用荧光定量pcr方法检测。
Methods the serum YMDD mutation, HBV DNA, ALT and HbeAg levels of 60 patients on lamivudine therapy were detected by FQ-PCR, ELISA, and rate method, respectively.
方法采用实时荧光定量pcr、ELISA及速率法分别检测60例乙肝患者经拉米呋啶治疗后其血清YMDD、HBVDNA、乙肝标志物及alt的变化情况。
Objective The serum HBV-DNA has been extensively detected by fluorescence-quantitative real time PCR (FQ-PCR) in our country.
目的目前国内较多的医院采用荧光定量的方法对血清中的HBV - DNA进行定量检测。
Results The positive rate of MTB detected with FQ-PCR was higher than that with smear acid-fast and culture of organism. The positive rate of non-tuberculosis was only 2.7%.
结果荧光定量pcr法检出结核杆菌阳性率显著高于痰涂片抗酸染色和培养法，其他非肺结核结果阳性率仅为2。
Results The positive rate of MTB detected with FQ-PCR was higher than that with smear acid-fast and culture of organism. The positive rate of non-tuberculosis was only 2.7%.
结果荧光定量pcr法检出结核杆菌阳性率显著高于痰涂片抗酸染色和培养法，其他非肺结核结果阳性率仅为2。
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