In the PCR reactions, the coneen- tration ratio between the degenerate primers was essential for influencing amplification efficiency.
在PCR反应中，简并引物之间的浓度配比是影响扩增效率的关键。
A pair of degenerate oligonucleotide primers named L2R2were synthesized according to conserved regions of the cytadhesin proteins from M. pneumonias. MG-S6DNA were used as model in L2R2 primered PCR.
根据肺炎支原体细胞粘附素蛋白质的基因保守区两侧序列，设计并合成一对变性寡核苷酸引物L2R2，用于以鸡毒支原体S6株基因组DNA为模板的PCR反应。
A pair of degenerate oligonucleotide primers named L2R2were synthesized according to conserved regions of the cytadhesin proteins from M. pneumonias. MG-S6DNA were used as model in L2R2 primered PCR.
根据肺炎支原体细胞粘附素蛋白质的基因保守区两侧序列，设计并合成一对变性寡核苷酸引物L2R2，用于以鸡毒支原体S6株基因组DNA为模板的PCR反应。
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